In the diagnosis and treatment of tuberculosis (tuberculosis, TB), especially multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis, the rapid and accurate identification of Mycobacterium tuberculosis and its drug resistance genes is crucial.The use of molecular biology techniques to detect drug-resistant genotypes of tuberculosis has become one of the diagnostic methods for drug-resistant tuberculosis.Compared with classic PCR and mNGS products,tNGS technologywere ableWide coverage of drug resistance genes and mutation sites of various anti-tuberculosis drugs, high sensitivity and good timeliness, can be used as a rapid screening method for drug-resistant tuberculosis and (or) an effective supplement to traditional drug susceptibility testing.
