Recently, the Department of Respiratory and Critical Care Medicine of Huai'an First People's Hospital Affiliated to Nanjing Medical University, in collaboration with Diffei Medical, published an article in Microbial Pathogenesis (IF=3.8) on the sputum of patients with different phenotypes of chronic obstructive pulmonary disease during acute exacerbation. Analytical study of microbial flora.This study collected sputum samples from 29 patients with different phenotypes of acute exacerbation of chronic obstructive pulmonary disease before treatment, used mNGS technology to analyze the differences in sputum microbial flora, and explored the relationship between sputum microbial flora and clinical symptoms in patients with acute exacerbation. The relationship between indicators has certain guiding significance for personalized treatment of AECOPD patients.

Recently, the Pathology Department of Beijing Chest Hospital Affiliated to Capital Medical University collaborated with Diffei Medical to publish an article in the Journal of Translational Medicine (IF=7.4).This study compared the performance of mNGS and conventional tuberculosis detection methods in 138 patients with pleural effusion. It also made full use of the human sequences and microbial sequences generated by mNGS to explore the value of this technology in infectious diseases and the identification of malignant tumors.

Hemophagocytic syndrome, also known as hemophagocytic lymphohistiocytosis (HLH).This disease is mainly caused by ineffective immunity leading to the activation of a large number of immune cells and the secretion of large amounts of cytokines, thereby causing progressive immune damage to the body.Usually, patients will have serious problems such as persistent fever, decreased blood count, hepatosplenomegaly, abnormal coagulation, and abnormal function of multiple organs. It is considered a critical disease with sudden onset, severe illness, rapid progression, and extremely high mortality rate. symptoms.Recently, the team of Deputy Director Bao Yongjian of the Department of Respiratory Medicine at Guangxi Jiangbin Hospital (Third People's Hospital of the Autonomous Region) cooperated with Difei Medical to perform mNGS testing on alveolar lavage fluid for a critically ill patient with repeated high fevers. The test results quickly identified EB virus infection. Hemophagocytic syndrome was diagnosed. After symptomatic treatment, the patient improved and was discharged from the hospital 11 days later and returned to school.The editor has compiled the cases as follows for clinical communication and learning.