The team of Professor Li Yuchuan from the Department of Infectious Diseases of the Children's Hospital of Fudan University collaborated with Diffei Medical to treat a case of Burkholderia pseudomallei sepsis in a child [1].The peripheral blood of the child was sent to Diffei Medical Laboratory for testing, and mNGS quickly identified Burkholderia pseudomallei.This case was Burkholderia pseudomallei sepsis in a child in a non-endemic area. The child's brother died from the same disease.Burkholderia pseudomallei sepsis is relatively rare in children and has a high mortality rate. Although it is rare in non-endemic areas, it should be highly valued clinically, and etiological examination should be completed as soon as possible and active anti-infective treatment should be given.This case has been included in the 'Chinese Clinical Case Results Database'.